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Encyclopedia > Wright's stain

Wright's stain is a technique in histology that is used to make the differences between cells visible under light microscopy. It is used in the examination of peripheral blood smears and bone marrow aspirates. Histology is the study of tissue sectioned as a thin slice, using a microscope. ... A microscopic view of an abnormal blood film. ...


It is named for James Homer Wright, who devised the stain, a modification of the Romanowsky stain, in 1902. Because it distinguishes easily between blood cells, it became widely used for performing differential white blood cell counts, which are routinely ordered when infections are expected, and as part of an assessment of overall health. Dr. James Homer Wright (1869-1928) was an early and influential American pathologist, who for many years was chief of pathology at Massachusetts General Hospital. ... Romanowsky staining was a prototypical staining technique that was the forerunner of several distinct but similar methods, including Giemsa, Wright, and Leishman stains, which are used to differentiate cells in pathologic specimens. ... White blood cells (also called leukocytes or immune cells) are a component of blood. ...


There are related stains known as the buffered Wright stain, the Wright-Giemsa stain, and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include Eosin Y, Azure B, and Methylene Blue (some commercial preparations combine solutions to simplify staining). The May-Grünwald Stain, which produces a more intense coloration, also takes a longer time to perform. Methylene Blue, methylthionine chloride or 3,7-bis(dimethylamino)phenothiazin-5-ium chloride is a chemical compound used as a dye and medication for Grams stain, Wrights stain, and Jenners stain and the treatment of methemoglobinemia. ...


 
 

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