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Encyclopedia > Triparental mating

Triparental mating is a form of Bacterial conjugation where a conjugative plasmid present in one bacterial strain assists the transfer of a mobilizable plasmid present in a second bacterial strain into a third bacterial strain. Plasmids are introduced into bacteria for such purposes as transformation, cloning, or transposon mutagenesis. Triparental matings can help overcome some of the barriers to efficient plasmid mobilization. For instance, if the conjugative plasmid and the mobilizable plasmid are members of the same incompatibility group they do not need to stably coexist in the second bacterial strain for the mobilizable plasmid to be transferred. Bacterial conjugation is the transfer of genetic material between bacteria through cell-to-cell contact. ... Figure 1: Schematic drawing of a bacterium with plasmids enclosed. ... It has been suggested that this article or section be merged into Transfection. ... Cloning is the process of creating an identical copy of something. ... Transposons are sequences of DNA that can move around to different positions within the genome of a single cell, a process called transposition. ...

Contents

Process

Requirements

  • A helper strain, Carrying a conjugative plasmid (such as the F-plasmid) that codes for genes required for conjugation and DNA transfer.
  • A donor strain, Carrying a mobilizable plasmid that can utilize the transfer functions of the conjugative plasmid.
  • A recipient strain, you wish to introduce the mobilizable plasmid into.

Five to seven days are required to determine if the plasmid was successfully introduced into the new bacterial strain and confirm that there is no carryover of the helper or donor strain. To meet Wikipedias quality standards, this article or section may require cleanup. ... Bacterial conjugation is the transfer of genetic material between bacteria through cell-to-cell contact. ... The structure of part of a DNA double helix Deoxyribonucleic acid (DNA) is a nucleic acid that contains the genetic instructions for the development and function of living organisms. ...


In contrast, Electroporation does not require a helper or donor strain. This helps avoid possible contamination with other strains. The introduction of the plasmid can be verified in the recipient strain in two days, making electroporation a faster and more efficient method of transformation. Electroporation, or electropermeabilization, is a significant increase in the electrical conductivity and permeability of the cell plasma membrane caused by externally applied electrical field. ...


See also

Bacterial conjugation is the transfer of genetic material between bacteria through cell-to-cell contact. ... Figure 1: Schematic drawing of a bacterium with plasmids enclosed. ... Transposons are sequences of DNA that can move around to different positions within the genome of a single cell, a process called transposition. ...

External links

References


 
 

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