Thermal cycler or thermocycler or PCR machine is a laboratory apparatus used for PCR. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then rises and lowers the temperature of the block in discreet, pre-programmed steps.
Modern thermal cyclers are often equipped with hot bonnet, a heated plate that presses against the lids of the reaction tubes. This prevents condensation of water from the reaction mixtures to the insides of the lids and makes it unnecessary to use PCR oil. Some thermal cyclers are equipped with multiple blocks allowing several different PCR reactions to be carried out simultaneously. Also some apparatuses have a gradient function, which allowes different temperatures in different parts of the block. This is particularly useful when testing suitable annealing temperatures for primers. The prizes for thermal cyclers start from USD 2500 (in 2004).
The chip is mounted on a one-by-three-inch plastic slide that provides the necessary mechanical, thermal, electrical and fluidic connections.
A sample size of 2-8 µl is used, PCR reactions are three times faster than conventional thermocyclers, and a portable, customized fluorescent-based optical reader analyzes the microarray in a few seconds.
The currently available platform hosts a pathogen panel to identify 10 sepsis-causing bacterial species as well as methicillin-resistant strains of Staphylococcus aureus from positive blood culture samples.
Share your thoughts, questions and commentary here
Want to know more? Search encyclopedia, statistics and forums:
Press Releases |
The Wikipedia article included on this page is licensed under the
Images may be subject to relevant owners' copyright.
All other elements are (c) copyright NationMaster.com 2003-5. All Rights Reserved.
Usage implies agreement with terms, 1022, m