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Encyclopedia > Restriction site

Restriction sites, or restriction recognition sites, are particular sequences of nucleotides that are recognized by restriction enzymes as sites to cut the DNA molecule. The sites are generally palindromic, and a particular enzyme may cut between two nucleotides within its recognition site, or somewhere nearby. For example, the common restriction enzyme EcoRI recognizes the sequence GAATTC and cuts between the G and the A on both the top and bottom strands, leaving an overhang (an end-portion of a DNA strand with no attached complement) on each end, of AATT. This overhang can then be used to ligate in (see DNA ligase) a piece of DNA with a complementary overhang (another EcoRI-cut piece, for example). A nucleotide is a chemical compound that consists of a heterocyclic base, a sugar, and one or more phosphate groups. ... A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded DNA. The enzyme makes two incisions, one through each of the phosphate backbones of the double helix without damaging the bases. ... The general structure of a section of DNA Deoxyribonucleic acid (DNA) is a nucleic acid —usually in the form of a double helix— that contains the genetic instructions specifying the biological development of all cellular forms of life, and most viruses. ... A palindrome is a word, phrase, number or any other sequence of units (like a strand of DNA) which has the property of reading the same in either direction (the adjustment of spaces between letters is generally permitted). ... In molecular biology, DNA ligase is a particular type of ligase (EC 6. ...


  Results from FactBites:
 
Exercise #4B - Physical Mapping of Recombinant Plasmid DNA by Digestion with Restriction Endonucleases (2067 words)
Restriction enzymes are site-specific endonucleases; that is, they cut DNA molecules only at sites where a specific sequence of bases occurs that the enzyme recognizes.
Restriction endonucleases are enzymes that cleave both strands of double stranded DNA after recognizing specific nucleotide sequences on the molecule.
Restriction enzymes "restrict" the host range of bacteriophage to strains of bacteria containing the identical set of restriction/modification genes present in the strain in which the virus was originally replicated.
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