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Encyclopedia > RecQ helicase

RecQ helicase is a family of helicase enzymes which unwind DNA for replication, transcription and repair. Mutations in the genes responsible for them are believed to be responsible for several disorders:

External links

  • RecQ Helicases (http://sekelsky.bio.unc.edu/Research/RecQ.html), introduction at UNC's Selsky Lab.
  • BLM gene encodes a RecQ Helicase (http://www.bio.davidson.edu/Courses/Molbio/MolStudents/spring2003/Baxter/BLMgene.html), descripton of the gene

  Results from FactBites:
 
ABBS 2005,37(09): RecQ Helicase-catalyzed DNA Unwinding Detected by Fluorescence Resonance Energy Transfer (3478 words)
The RecQ family helicases BLM and WRN are mutated in Bloom's syndrome and Werner's syndrome, respectively, and RecQ4 is defective in Rothmund-Thomson syndrome.
RecQ protein was pre-incubated with fluorescent-labeled dsDNA substrate in unwinding buffer at 25 ºC, and the emission spectrum was measured.
The dsDNA substrate and RecQ helicase were mixed and incubated at 25 ºC for 5 min in unwinding buffer A in syringe 1 and the unwinding reaction was initiated by rapid mixing with ATP from syringe 4.
  More results at FactBites »

 
 

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