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Encyclopedia > Pyruvate decarboxylase

Pyruvate decarboxylase is a homotetrameric enzyme(EC 4.1.1.1) that catalyses the decarboxylation of pyruvic acid to acetaldehyde and carbon dioxide. A tetramer is a protein with four subunits (tetrameric). ... Ribbon diagram of the enzyme TIM, surrounded by the space-filling model of the protein. ... The Enzyme Commission number (EC number) is a numerical classification scheme for enzymes, based on the chemical reactions they catalyze. ... In chemistry and biology, catalysis is the acceleration (increase in rate) or slowing down of a chemical reaction by means of a substance, called a catalyst, that is itself not consumed by the overall reaction. ... Pyruvic acid (CH3COCO2H) is an alpha-keto acid which plays an important role in biochemical processes. ... R-phrases , , S-phrases , , , Flash point −39 °C Autoignition temperature 185 °C RTECS number AB1925000 Supplementary data page Structure and properties n, εr, etc. ... Carbon dioxide is a chemical compound composed of one carbon and two oxygen atoms. ...


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Chapter 2 Pyruvate decarboxylase: An indispensable enzyme for growth of Saccharomyces cerevisiae on glucose (5442 words)
For induction of pyruvate decarboxylase, 10 ml of a preculture was inoculated in a 100 ml shake flask with either 50 ml YPE (2% ethanol) or 50 ml YPD (8% glucose) and shaken for 6 hours at 30 °C (Hohmann, 1991a).
The wash-out of biomass and the accumulation of glucose was accompanied by the transient accumulation of pyruvate in the culture to a maximum concentration of 7 mM (Fig.
Pyruvate decarboxylase, acetaldehyde dehydrogenase and acetyl-CoA synthetase all have been reported to be present in the cytosol of wild-type S.
Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes - Patent 5000000 (9392 words)
Pyruvate decarboxylase is the key enzyme in this pathway which diverts the flow of pyruvate to ethanol.
During normal fermentative growth, pyruvate is converted to acetyl coenzyme A by the pyruvate dehydrogenase complex, to oxaloacetate (and on to succinate) by phosphoenolpyruvate carboxylase, to formate and acetyl coenzyme by pyruvate formate lyase, and to lactate by lactate dehydrogenase.
Pyruvate decarboxylase activity.sup.a ATCC Strain pLOI297 pLOI308-11 ______________________________________ 8677 5.7 6.0 8739 0.8 1.4 9637 1.1 1.4 11303 16.7 2.1 11775 17.1 --.sup.b 14948 --.sup.b 2.5 15224 16.3 1.8 23227 2.3 1.7 ______________________________________.sup.a Activity in I.U./mg cell protein.
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