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Encyclopedia > Protein sequence

Peptide sequence or amino acid sequence is the order in which amino acid residues, connected by peptide bonds, lie in the chain. The sequence is generally reported from the N-terminal end containing free amino group to the C-terminal end containing free carboxyl group. Peptide sequence is often called protein sequence if it represent the primary structure of a protein.

Several deductions can be made from the sequence. Long stretches of hydrophobic residues may indicate transmembrane helices. Certain residues indicate a beta sheet area. If full-lenght protein sequence is available, it is possible to estimate the isoelectric point of the protein. Methods for determining the peptide sequence include deduction from DNA sequence, Edman degradation, and mass spectrometry.

  Results from FactBites:
Protein - Wikipedia, the free encyclopedia (5445 words)
Proteins are polymers whose amino acid sequence is specified by a gene encoded in the genetic code.
Protein is also a necessary component in the diet of humans and other animals that cannot synthesise all amino acids and must obtain essential amino acids from their diet.
Consequently, numerous alternative theories of the protein primary structure were proposed, e.g., the colloidal hypothesis that proteins were assemblies of small molecules, the cyclol hypothesis of Dorothy Wrinch, the diketopiperazine hypothesis of Emil Abderhalden and the pyrrol/piperidine hyopthesis of Troensgard (1942).
Protein sequencing - Wikipedia, the free encyclopedia (1778 words)
Proteins are found in every cell and are essential to every biological process, protein structure is very complex: determining a protein's structure involves first protein sequencing - determining the amino acid sequences of its constituent peptides; and also determining what conformation it adopts and whether it is complexed with any non-peptide molecules.
Discovering the structures and functions of proteins in living organisms is an important tool for understanding cellular processes, and allows drugs that target specific metabolic pathways to be invented more easily.
The amino acid sequence of a protein can also be determined indirectly from the mRNA or, in organisms such as prokaryotes that do not have introns the DNA that codes for the protein.
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