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Encyclopedia > Nirenberg and Leder experiment

The Nirenberg and Leder experiment was a scientific experiment performed in 1964 by Marshall W. Nirenberg and Philip Leder. The experiment elucidated the triplet nature of the genetic code and allowed the codons of the standard genetic code to be deciphered. Marshall Nirenberg won a Nobel Prize in 1968 Marshall Warren Nirenberg (born April 10, 1927) was a U.S. biochemist and geneticist. ... RNA codons. ... RNA codons. ...


In the experiment, various combinations of mRNA were passed through a filter which contained ribosomes. Unique triplets promoted the binding of specific tRNAs to the ribosome. By associating the tRNA with its specific amino acid, it was possible to determine the triplet mRNA sequence that coded for each amino acid. The interaction of mRNA in a eukaryote cell. ... Figure 1: Ribosome structure indicating small subunit (A) and large subunit (B). ... Transfer RNA (abbreviated tRNA) is a small RNA chain (74-93 nucleotides) that transfers a specific amino acid to a growing polypeptide chain at the ribosomal site of protein synthesis during translation. ... In chemistry, an amino acid is any molecule that contains both amino and carboxylic acid functional groups. ...


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  Results from FactBites:
 
Nirenberg and Matthaei experiment - Encyclopedia, History, Geography and Biography (196 words)
The Nirenberg and Matthaei experiment was a scientific experiment performed in 1961 by Marshall W. Nirenberg and Heinrich J. Matthaei.
The experiment cracked the genetic code by using nucleic acid homopolymers to translate specific amino acids.
In the experiment, an extract from bacterial cells that could make protein even when no intact living cells were present was prepared.
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