Under alkaline conditions the divalent copper ion forms a complex with peptide bonds in which it is reduced to a monovalent ion. Monovalent copper ion and the radical groups of tyrosine, tryptophan, and cysteine react with Folin reagent to produce an unstable product that becomes reduced to molybdenum/tungsten blue.
An assay is a procedure where the concentration of a component part of a mixture is determined.
There are numerous applications of an assay, such as an antigen capture assay, bioassay, competitive protein binding assay, four-point assay, immunoassay, microbiological assay, stem cell assay, and many others.
Assays are regularly utilized in scientific research laboratories.
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