In 1998 he was a Payload Specialist aboard NASA Space Shuttle flight STS-90 as part of the Neurolab mission from April 17 to May 3, 1998. Aboard the Neurolab Mission, Buckey was the Payload Specialist for the experiment "Cardiovascular Adaptation to Zero-Gravity" and assisted with other Spacelab Life Sciences experiments. During the 16-day Spacelab flight, the seven person crew aboard Space Shuttle Columbia served as both experiment subjects and operators for 26 individual life science experiments focusing on the effects of microgravity on the brain and nervous system. The STS-90 flight orbited the Earth 256 times, covered 6.3 million miles, and logged him over 381 hours in space.
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