JC was first isolated from the brain from a patient suffering from Hodgkin's disease, and suffering from PML.
Polyomavirus replication in permissive cells can be detected by observing CPE or plaque production, by identifying virus particles in cell or culture fluid by EM, by detecting virus antigens using IF, or by observing haemagglutination.
JC and BK viruses are ubiquitous throughout the world, and the 2 viruses circulate independently.
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