FACTOID # 5: Minnesota and Connecticut are both in the top 5 in saving money and total tax burden per capita.
 
 Home   Encyclopedia   Statistics   States A-Z   Flags   Maps   FAQ   About 
 
WHAT'S NEW
 

SEARCH ALL

FACTS & STATISTICS    Advanced view

Search encyclopedia, statistics and forums:

 

 

(* = Graphable)

 

 


Encyclopedia > Intracytoplasmic sperm injection
Oocyte is injected during ICSI
Oocyte is injected during ICSI

Intracytoplasmic sperm injection (ICSI) is an in vitro fertilization procedure in which a single sperm is injected directly into an egg; this procedure is most commonly used to overcome male infertility problems. Image File history File links ICSI sperm injection into oocyte File history Legend: (cur) = this is the current file, (del) = delete this old version, (rev) = revert to this old version. ... Image File history File links ICSI sperm injection into oocyte File history Legend: (cur) = this is the current file, (del) = delete this old version, (rev) = revert to this old version. ... In vitro fertilization (IVF) is a technique in which egg cells are fertilized outside the mothers body in cases where conception is difficult or impossible through normal intercourse. ... A spermatozoon or spermatozoan ( spermatozoa), from the ancient Greek σπέρμα (seed) and (living being) and more commonly known as a sperm cell, is the haploid cell that is the male gamete. ... A human ovum Sperm cells attempting to fertilize an ovum An ovum (plural ova) is a haploid female reproductive cell or gamete. ... Infertility primarily refers to the biological inability of a man or a woman to contribute to conception. ...


The procedure is done under a microscope using micromanipulation devices (micromanipulators, microinjectors and micropipettes). A holding pipette (on picture on left) stablizes the mature oocyte. From the opposite site a thin, hollow needle is pierced through the oolemma and into the inner part of the oocyte. It is loaded with a single sperm that will be released into the oocyte. The pictured oocyte has an extruded polar body at about 12 o'clock indicating its maturity. After the procedure, the oocyte will be placed into cell culture and checked on the following day for signs of fertilization. Microinjector This device provides the pressure which is needed to deliver the sample solution from the micropipette into the cells. ... Micropipette Micropipettes are microtools constructed from glass tubings for microinjection and micromanipulation purposes. ... An oocyte or ovocyte is a female gametocyte or germ cell involved in reproduction. ... Polar body is a cell structure found inside an ovum. ... Categories: Biology stubs ...


In natural fertilization sperm compete and when the first sperm enters the egg cell, the egg cell blocks the entry of any other sperm. Concern has been raised that in ICSI this sperm selection process is bypassed and the sperm is selected by the embryologist without any specific testing. However, in mid 2006 the FDA cleared a device that allows embryologists to select mature sperm for ICSI based on sperm binding to hyaluronan, the main constituent of the gel layer (cumulus oophorus) surrounding the oocyte. The device provides microscopic droplets of hyaluronan hydrogel attached to the culture dish. The embryologist places the prepared sperm on the microdot, selects and captures sperm that bind to the dot. Basic research on the maturation of sperm shows that hyaluronan-binding sperm are more mature and show fewer DNA strand breaks and significantly lower levels of aneuploidy than the sperm population from which they were selected. The repeating disaccharide unit of hyaluronan Hyaluronan (also called hyaluronic acid or hyaluronate) is a non-sulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissues. ... At one part of the mature follicle the cells of the membrana granulosa are collected into a mass which projects into the cavity of the follicle. ...


There is some suggestion that birth defects are increased with the use of IVF in general, and ICSI specifically. It is hoped that use of new device (PICSI) will lower the apparently elevated level of birth defects associated with ICSI.(see section of IVF discussing birth defects) A congenital disorder is a medical condition or defect that is present at or before birth (for example, congenital heart disease). ... In vitro fertilization (IVF) is a technique in which egg cells are fertilized outside the mothers body in cases where conception is difficult or impossible through normal intercourse. ...


See also

  • Reproductive technology
  • The Epigenome Network of Excellence (NoE)
  • The Human Fertilisation and Embryology Authority (HFEA)

  Results from FactBites:
 
Intracytoplasmic sperm injection - Wikipedia, the free encyclopedia (233 words)
Intracytoplasmic sperm injection (ICSI) is an in vitro fertilization procedure in which a single sperm is injected directly into an egg; this procedure is most commonly used to overcome male infertility problems.
In natural fertilization sperm compete and when the first sperm enters the egg cell, the egg cell blocks the entry of any other sperm.
Concern has been raised that in ICSI this sperm selection process is bypassed and the sperm is selected by the embryologist without undergoing any specific testing.
TCART - Intracytoplasmic Sperm Injection (312 words)
ICSI is the acronym to describe Intracytoplasmic Sperm Injection, the injection of a single sperm directly into the cytoplasm of an oocyte.
The sperm suspension is kept at 37°C in an incubator until the moment of injection.
Prior to injection, the sperm is specially prepared and immobilized using an extremely viscous solution of polyvinylpyrrolidone (PVP), enabling the technologist to aspirate a single sperm for injection directly into the cytoplasm of the oocyte.
  More results at FactBites »

 
 

COMMENTARY     


Share your thoughts, questions and commentary here
Your name
Your comments

Want to know more?
Search encyclopedia, statistics and forums:

 


Press Releases |  Feeds | Contact
The Wikipedia article included on this page is licensed under the GFDL.
Images may be subject to relevant owners' copyright.
All other elements are (c) copyright NationMaster.com 2003-5. All Rights Reserved.
Usage implies agreement with terms, 1022, m