A Fluorescence Microscope is a light microscope used to study properties of organic or inorganic substances using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption. Jump to: navigation, search 1852 microscope Compound microscope made by John Cuff in 1750 A microscope (Greek: micron = small and scopos = aim) is an instrument for viewing objects that are too small to be seen by the naked or unaided eye. ... Fluorescence induced by exposure to ultraviolet light in vials containing various sized cadmium selenide (CdSe) quantum dots. ... Phosphorescent powder under visible light, ultraviolet light, and total darkness. ... The word reflection (also spelt reflexion in British English) can refer to several different concepts: In mathematics, reflection is the transformation of a space. ... Absorption has a number of meanings: In physics, absorption is a process in which particles of some sort encounter another material and are taken up by or even disappear in it. ...
Most fluorescence microscopes in use are Epi-Fluorescence Microscopes (ie : excitation and observation of the fluorescence are from above (epi) the specimen). These microscopes have become an important part in the field of biology, opening the doors for more advanced microscope designs, such as Confocal laser scanning microscope and the Total internal reflection fluorescence microscope. Confocal laser scanning microscopy (CLSM or LSCM) is a valuable tool for obtaining high resolution images and 3-D reconstructions. ... Principle of the TIRFM A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nm, can be observed. ...
Fluorescence is the property of some atoms and molecules to absorb light at a particular wavelength and to subsequently emit light of longer wavelength after a brief interval, termed the fluorescence lifetime.
Fluorescence and Differential Interference Contrast Combination Microscopy - Fluorescence microscopy can also be combined with contrast enhancing techniques such as differential interference contrast (DIC) illumination to minimize the effects of photobleaching by locating a specific area of interest in a specimen using DIC then, without relocating the specimen, switching the microscope to fluorescence mode.
Fluorescence Microscopy of Cells in Culture - Serious attempts at the culture of whole tissues and isolated cells were first undertaken in the early 1900s as a technique for investigating the behavior of animal cells in an isolated and highly controlled environment.
The basic function of a fluorescencemicroscope is to irradiate the specimen with a desired and specific band of wavelengths, and then to separate the much weaker emitted fluorescence from the excitation light.
Modern fluorescencemicroscopes are capable of accommodating between four and six fluorescence cubes (usually on a revolving turret or through a slider mechanism; see Figure 1) and permit the user to easily attach replacement aftermarket excitation and barrier filters, as well as dichromatic mirrors.
Fluorescence vertical illuminators are designed with the purpose of controlling the excitation light through the application of readily interchangeable filter (neutral density and interference excitation balancers) insertions into the light path on the way toward the specimen, and again in the path between the specimen and the observation tubes or camera detector system.
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