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Encyclopedia > Deoxycytidine triphosphate

Deoxycytidine triphosphate (dCTP) is a cytidine nucleotide triphosphate that is used whenever DNA is synthesized, such as in the polymerase chain reaction. e.g.: Cytidine is a molecule (known as a nucleoside) that is formed when cytosine is attached to a ribose ring (also known as a ribofuranose) via a β-N1-glycosidic bond. ... A nucleotide is a chemical compound that consists of a heterocyclic base, a sugar, and one or more phosphate groups. ... In inorganic chemistry, a phosphate is a salt of phosphoric acid. ... The general structure of a section of DNA Deoxyribonucleic acid (DNA) is a nucleic acid — usually in the form of a double helix — that contains the genetic instructions or genocode monitoring the biological development of all cellular forms of life, and many viruses. ... Polymerase chain reaction (PCR) is a molecular biology technique, invented in 1985 by Kary B. Mullis,[1] for enzymatically replicating DNA without using a living organism, such as E. coli or yeast. ...

A chemical equation can be written that represents the process: A chemical equation is a symbolic representation of a chemical reaction. ...

(DNA)n + dCTP ↔ (DNA)n-C + PPi

That is, dCTP has the PPi (pyrophosphate) cleaved off and the dCMP is incorporated into the DNA strand at the 3' end. In chemistry, the anion, the salts, and the esters of pyrophosphoric acid are called pyrophosphates. ...

It is stored at -20 degrees Celsius. A degree Celsius (°C) is a unit of temperature named after the Swedish astronomer Anders Celsius (1701-1744), who first proposed a similar system in 1742. ...

See also

DNA Replication DNA replication. ...

  Results from FactBites:
Cytidine Triphosphate (CTP): a nucleotide, the 5'-triphosphate of cytidine; precursor in the synthesis of ribonucleic acid and of CDP- and CMP-linked compounds.
Deoxycytidine Monophosphate (dCMP): a nucleotide, the 5'-phosphate of deoxycytidine (deoxy-, also called desoxy, is a prefix for the designation of compounds which contain one less atom of oxygen than the reference substance).
Deoxycytidine Triphosphate (dCTP): a nucleotide, the 5'-triphosphate of deoxycytidine; an precursor in the synthesis of DNA.
Attotron Molecular Biology Hypertext Dictionary (9690 words)
ddNTP.GIF a nucleoside triphosphate analog that causes termination of an elongating DNA molecule when incorporated by DNA polymerase because of the absence of a 3´-hydroxyl residue on the sugar portion of the compound, which is necessary for the formation of the 3' to 5' phosphate linkage in an elongating DNA chain.
The sugar molecules are modified by the presence of a purine or pyrimidine nucleoside or "base" attached to the first, or 1', carbon and the oxygen-phosphorus-oxygen bond links the third, or 3', carbon to the fifth, or 5', carbon of the adjacent sugar molecule.
thymidine triphosphate, a deoxyribose molecule with the pyrimidine thymine attached to the 1´ carbon and a chain of three oxygenated phosphorus atoms attached to the 5' carbon.
  More results at FactBites »



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