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Encyclopedia > DNA sequencers

A DNA sequencer is a machine to automatize the DNA sequencing process. Wind turbines A machine is any mechanical or organic device that transmits or modifies energy to perform or assist in the performance of tasks. ... DNA sequencing is the process of determining the nucleotide order of a given DNA fragment, called the DNA sequence. ...


In time of big genomes projects, to enhance human productivity is important.


Modern automated DNA sequencing instruments (called DNA sequencers) are able to sequence as many as 384 fluoresecently labelled samples in a batch (run) and perform as many as 24 runs a day. These perform only the size separation and peak reading; the actual sequencing reaction(s), cleanup and resuspension in a suitable buffer must be performed separately.


The magnitude of the fluorescent signal is realated to the number of strands of DNA that are in the reaction. If the initial amount of DNA is small, the signals will be weak. However, the properties of PCR allow one to increase the signal by increasing the number of cycles in the PCR programme.


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  Results from FactBites:
 
DNA analysis (641 words)
First, the basis for virtually all DNA sequencing was the dideoxy-chain terminating reaction, developed by Sanger and for which he received his second Nobel prize.
DNA polymerase and deoxynucleotides are added to the mixture, and the enzyme catalyzes the synthesis of the complementary DNA strand, starting at the primer and extending in the 3' direction.
When the sequencing reaction proceeds in the presence of a small amount of the dideoxynucleotide, the resulting chain-extension reaction products will consist of a set of molecules, all starting at the primer, all extending in the 3' direction, but terminating wherever the dideoxynucleotide happened to be incorporated, opposite its complementary nucleotide in the template.
Sequencer - Wikipedia, the free encyclopedia (283 words)
Sequencers started off in 1992 with a debut Album "Sahara" that was a major hit.
Sequencers got low profile from year 1996 due to the slump of music industry in Pakistan.
Now since couple of years media promotion has been shot out with launch of many private and international satellite channels that after eight years SEQUENCERS are planning for a comeback.
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