A DNA sequencer is a machine to automatize the DNA sequencing process. Wind turbines A machine is any mechanical or organic device that transmits or modifies energy to perform or assist in the performance of tasks. ... DNA sequencing is the process of determining the nucleotide order of a given DNA fragment, called the DNA sequence. ...
In time of big genomes projects, to enhance human productivity is important.
Modern automated DNA sequencing instruments (called DNA sequencers) are able to sequence as many as 384 fluoresecently labelled samples in a batch (run) and perform as many as 24 runs a day. These perform only the size separation and peak reading; the actual sequencing reaction(s), cleanup and resuspension in a suitable buffer must be performed separately.
The magnitude of the fluorescent signal is realated to the number of strands of DNA that are in the reaction. If the initial amount of DNA is small, the signals will be weak. However, the properties of PCR allow one to increase the signal by increasing the number of cycles in the PCR programme.
Category: Molecular biology PerkinElmer, Inc. ... Beckman Coulter Inc. ... 454 Life Sciences is a biotechnology company based in Branford, Connecticut specializing in high-throughput DNA sequencing using a novel massively parallel sequencing-by-synthesis approach. ...
First, the basis for virtually all DNAsequencing was the dideoxy-chain terminating reaction, developed by Sanger and for which he received his second Nobel prize.
DNA polymerase and deoxynucleotides are added to the mixture, and the enzyme catalyzes the synthesis of the complementary DNA strand, starting at the primer and extending in the 3' direction.
When the sequencing reaction proceeds in the presence of a small amount of the dideoxynucleotide, the resulting chain-extension reaction products will consist of a set of molecules, all starting at the primer, all extending in the 3' direction, but terminating wherever the dideoxynucleotide happened to be incorporated, opposite its complementary nucleotide in the template.
Share your thoughts, questions and commentary here
Want to know more? Search encyclopedia, statistics and forums:
Press Releases |
The Wikipedia article included on this page is licensed under the
Images may be subject to relevant owners' copyright.
All other elements are (c) copyright NationMaster.com 2003-5. All Rights Reserved.
Usage implies agreement with terms, 1022, m