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Encyclopedia > Aesculin

Aesculin is a glucoside that naturally occurs in the horse chestnut Aesculus hippocastanum and in daphnin (the dark green resin of Daphne mezereum). A glucoside is a glycoside that is derived from glucose. ... Species Aesculus arguta: Texas Buckeye Aesculus californica: California Buckeye Aesculus chinensis: Chinese Horse-chestnut Aesculus flava (): Yellow Buckeye Aesculus glabra: Ohio Buckeye Aesculus hippocastanum: Common Horse-chestnut Aesculus indica: Indian Horse-chestnut Aesculus neglecta: Dwarf Buckeye Aesculus parviflora: Bottlebrush Buckeye Aesculus pavia: Red Buckeye Aesculus sylvatica: Painted Buckeye Aesculus turbinata... Binomial name Daphne mezereum L. Daphne mezereum is a species of Daphne in the flowering plant family Thymelaeaceae, native to most of Europe and western Asia, north to northern England and central Scandinavia. ...


Medical uses

Aesculin is used in a microbiology laboratory to aid in the identification of bacterial species (especially Enterococci). Species E. faecalis etc. ...


Aesculin hydrolysis test

Aesculin is incorporated into agar with ferric citrate and bile salts (bile aesculin agar). Hydrolysis of the aesculin forms aesculetin (6,7-dihydroxycoumarin) and glucose. The aesculetin forms dark brown or black complexes with ferric citrate, allowing the test to be read. Coumarin is a chemical compound found in many plants, notably in high concentration in the tonka bean, woodruff, and bison grass. ...


Streak the bile aesculin agar and incubate at 37°C for 24 hours. The presence of a dark brown or black halo indicates that the test is positive. A positive test can occur with Enterococcus, Aerococcus and Leuconostoc. Aesculin will fluoresce under long wave ultraviolet light (360nm): hydrolysis of aesculin results in loss of this fluorescence. Species E. faecalis etc. ... Leuconostoc is a kind of bacteria, often found in butter. ... Fluorescence induced by exposure to ultraviolet light in vials containing various sized cadmium selenide (CdSe) quantum dots. ... UV redirects here. ...


Enterococcus will often flag positive within four hours of the agar being inoculated.


References

  • See National Standard Methods MSOP 48 (Bile aesculin agar) and BSOPTP 2 (Aesculin hydrolysis test).

 
 

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